Title:Active regulation of GATAs and how these transcription factors drive cell activation kinetics, phenotype, and polarization
Abstract: GATA3 and GATA6 biosensors were developed and used to investigate transcription factors upstream of the myeloid response transcriptome and how the regulation of these factors affects the composition of macrophage population. Specifically, the activation of GATA6 will be monitored during macrophage differentiation and injury response in RAW264.7 cells in order to investigate how GATA6 is driving macrophage functions. Additionally, the activity of GATA6 will also be monitored during the differentiation of bone marrow isolates into macrophages in M-CSF over time. These biosensor tools enable a better understand biological pathways and determine where pharmacological intervention may be possible.
